Unknown

Dataset Information

0

Development of Folate-Functionalized PEGylated Zein Nanoparticles for Ligand-Directed Delivery of Paclitaxel.


ABSTRACT: In this study, we investigated the active targeted delivery of a hydrophobic drug, paclitaxel (PTX), via receptor-mediated endocytosis by folate receptors expressed on cancer cells using a protein-based nanoparticle system. PTX was loaded on zein nanoparticles and conjugated with folate (PTX/Zein-FA) to estimate its chemotherapeutic efficacy in folate receptor-expressing KB cancer cells. PTX/Zein-FA nanoparticles were successfully developed, with a nanoparticle size of ~180 nm and narrow polydispersity index (~0.22). Accelerated release of PTX in an acidic environment was observed for PTX/Zein-FA. An in vitro cellular study of PTX/Zein-FAs in KB cells suggested that PTX/Zein-FA improved the cytotoxic activity of PTX on folate receptors overexpressed in cancer cells by inducing proapoptotic proteins and inhibiting anti-apoptotic proteins. In addition, PTX/Zein-FA exhibited anti-migratory properties and could alter the cell cycle profile of KB cells. A549 cells, which are folate receptor-negative cancer cells, showed no significant enhancement in the in vitro cellular activities of PTX/Zein-FA. We describe the antitumor efficacy of PTX/Zein-FA in KB tumor-bearing mice with minimum toxicity in healthy organs, and the results were confirmed in comparison with free drug and non-targeted nanoparticles.

SUBMITTER: Soe ZC 

PROVIDER: S-EPMC6920870 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Development of Folate-Functionalized PEGylated Zein Nanoparticles for Ligand-Directed Delivery of Paclitaxel.

Soe Zar Chi ZC   Ou Wenquan W   Gautam Milan M   Poudel Kishwor K   Kim Bo Kyun BK   Pham Le Minh LM   Phung Cao Dai CD   Jeong Jee-Heon JH   Jin Sung Giu SG   Choi Han-Gon HG   Ku Sae Kwang SK   Yong Chul Soon CS   Kim Jong Oh JO  

Pharmaceutics 20191030 11


In this study, we investigated the active targeted delivery of a hydrophobic drug, paclitaxel (PTX), via receptor-mediated endocytosis by folate receptors expressed on cancer cells using a protein-based nanoparticle system. PTX was loaded on zein nanoparticles and conjugated with folate (PTX/Zein-FA) to estimate its chemotherapeutic efficacy in folate receptor-expressing KB cancer cells. PTX/Zein-FA nanoparticles were successfully developed, with a nanoparticle size of ~180 nm and narrow polydis  ...[more]

Similar Datasets

| S-EPMC5551526 | biostudies-literature
| S-EPMC6958489 | biostudies-literature
| S-EPMC4423828 | biostudies-literature
| S-EPMC10545574 | biostudies-literature
| S-EPMC2948946 | biostudies-literature
| S-EPMC4168894 | biostudies-literature
| S-EPMC8011395 | biostudies-literature
| S-EPMC9332751 | biostudies-literature
| S-EPMC3147305 | biostudies-literature
| S-EPMC5796468 | biostudies-literature