Ontology highlight
ABSTRACT:
SUBMITTER: Yang D
PROVIDER: S-EPMC6921666 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Yang Dong D Li Shaoji S Stabenow Jennifer J Zalduondo Lillian L Kong Ying Y
Infection and immunity 20191217 1
<i>Mycobacterium tuberculosis</i> Rv3775 (LipE) was annotated as a putative lipase. However, its lipase activity has never been characterized, and its precise role in tuberculosis (TB) pathogenesis has not been thoroughly studied to date. We overexpressed and purified the recombinant LipE (rLipE) protein and demonstrated that LipE has a lipase/esterase activity. rLipE prefers medium-chain ester substrates, with the maximal activity on hexanoate. Its activity is the highest at 40°C and pH 9. We d ...[more]