Dataset Information

High-throughput sequencing of CD4⁺ T cell repertoire reveals disease-specific signatures in IgG4-related disease.

ABSTRACT:

Background

CD4⁺ T cells play critical roles in the pathogenesis of IgG4-related disease (IgG4-RD). The aim of this study was to investigate the TCR repertoire of peripheral blood CD4⁺ T cells in IgG4-RD.

Methods

The peripheral blood was collected from six healthy controls and eight IgG4-RD patients. TCR β-chain libraries of CD4⁺ T cells were constructed by 5'-rapid amplification of cDNA ends (5'-RACE) and sequenced by Illumina Miseq platform. The relative similarity of TCR repertoires between samples was evaluated according to the total frequencies of shared clonotypes (metric F), correlation of frequencies of shared clonotypes (metric R), and total number of shared clonotypes (metric D).

Results

The clonal expansion and diversity of CD4⁺ T cell repertoire were comparable between healthy controls and IgG4-RD patients, while the proportion of expanded and coding degenerated clones, as an indicator of antigen-driven clonal expansion, was significantly higher in IgG4-RD patients. There was no significant difference in TRBV and TRBJ gene usage between healthy controls and IgG4-RD patients. The complementarity determining region 3 (CDR3) length distribution was skewed towards longer fragments in IgG4-RD. Visualization of relative similarity of TCR repertoires by multi-dimensional scaling analysis showed that TCR repertoires of IgG4-RD patients were separated from that of healthy controls in F and D metrics. We identified 11 IgG4-RD-specific CDR3 amino acid sequences that were expanded in at least 2 IgG4-RD patients, while not detected in healthy controls. According to TCR clonotype networks constructed by connecting all the CDR3 sequences with a Levenshtein distance of 1, 3 IgG4-RD-specific clusters were identified. We annotated the TCR sequences with known antigen specificity according to McPAS-TCR database and found that the frequencies of TCR sequences associated with each disease or immune function were comparable between healthy controls and IgG4-RD patients.

Conclusion

According to our study of CD4⁺ T cells from eight IgG4-RD patients, TCR repertoires of IgG4-RD patients were different from that of healthy controls in the proportion of expanded and coding degenerated clones and CDR3 length distribution. In addition, IgG4-RD-specific TCR sequences and clusters were identified in our study.

SUBMITTER: Wang L

PROVIDER: S-EPMC6923942 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Dataset Information

High-throughput sequencing of CD4⁺ T cell repertoire reveals disease-specific signatures in IgG4-related disease.

Background

Methods

Results

Conclusion

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OmicsDI is part of the ELIXIR infrastructure

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High-throughput sequencing of CD4+ T cell repertoire reveals disease-specific signatures in IgG4-related disease.

Background

Methods

Results

Conclusion

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OmicsDI is part of the ELIXIR infrastructure

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High-throughput sequencing of CD4⁺ T cell repertoire reveals disease-specific signatures in IgG4-related disease.