C. elegans expressing D76N ?₂-microglobulin: a model for in vivo screening of drug candidates targeting amyloidosis.

ABSTRACT: The availability of a genetic model organism with which to study key molecular events underlying amyloidogenesis is crucial for elucidating the mechanism of the disease and the exploration of new therapeutic avenues. The natural human variant of ?₂-microglobulin (D76N ?₂-m) is associated with a fatal familial form of systemic amyloidosis. Hitherto, no animal model has been available for studying in vivo the pathogenicity of this protein. We have established a transgenic C. elegans line, expressing the human D76N ?₂-m variant. Using the INVertebrate Automated Phenotyping Platform (INVAPP) and the algorithm Paragon, we were able to detect growth and motility impairment in D76N ?₂-m expressing worms. We also demonstrated the specificity of the ?₂-m variant in determining the pathological phenotype by rescuing the wild type phenotype when ?₂-m expression was inhibited by RNA interference (RNAi). Using this model, we have confirmed the efficacy of doxycycline, an inhibitor of the aggregation of amyloidogenic proteins, in rescuing the phenotype. In future, this C. elegans model, in conjunction with the INVAPP/Paragon system, offers the prospect of high-throughput chemical screening in the search for new drug candidates.

SUBMITTER: Faravelli G 

PROVIDER: S-EPMC6934621 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature