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N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells.

ABSTRACT: SGK196 is a protein O-mannose kinase involved in an indispensable phosphorylation step during laminin-binding glycan synthesis on alpha-dystroglycan (?-DG). However, the function of SGK196 in cancer diseases remains elusive. In the current study, we demonstrated that SGK196 is primarily modified by N-glycosylation in breast cancer (BC) cells. Furthermore, gain and loss-of-function studies showed that N-glycosylated SGK196 suppresses cell migration, invasion, and metastasis in BC, particularly in the basal-like breast cancer (BLBC) type. In addition, we found that SGK196 N-glycosylation performs the regulatory function through the PI3K/AKT/GSK3? signaling pathway. Collectively, our results show that N-glycosylated SGK196 plays suppression roles in BLBC metastases, therefore providing new insights into SGK196 function in BC.

SUBMITTER: Xu C

PROVIDER: S-EPMC6949223 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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N-glycosylated SGK196 suppresses the metastasis of basal-like breast cancer cells.

Xu Ci C Zhang Meichao M Bian Lei L Li Yanyan Y Yao Yuan Y Li Dong D

Oncogenesis 20200108 1

SGK196 is a protein O-mannose kinase involved in an indispensable phosphorylation step during laminin-binding glycan synthesis on alpha-dystroglycan (α-DG). However, the function of SGK196 in cancer diseases remains elusive. In the current study, we demonstrated that SGK196 is primarily modified by N-glycosylation in breast cancer (BC) cells. Furthermore, gain and loss-of-function studies showed that N-glycosylated SGK196 suppresses cell migration, invasion, and metastasis in BC, particularly in ...[more]

PMID: 31913260

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