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Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication.

ABSTRACT: Anthocyanins protect plants from biotic and abiotic stressors and provide great health benefits to consumers. In this study, we cloned four genes (Red Lettuce Leaves 1 to 4: RLL1 to RLL4) that contribute to colour variations in lettuce. The RLL1 gene encodes a bHLH transcription factor, and a 5-bp deletion in some cultivars abolishes its function to activate the anthocyanin biosynthesis pathway. The RLL2 gene encodes an R2R3-MYB transcription factor, which was derived from a duplication followed by mutations in its promoter region. The RLL3 gene encodes an R2-MYB transcription factor, which down-regulates anthocyanin biosynthesis through competing with RLL2 for interaction with RLL1; a mis-sense mutation compromises the capacity of RLL3 to bind RLL1. The RLL4 gene encodes a WD-40 transcription factor, homologous to the RUP genes suppressing the UV-B signal transduction pathway in Arabidopsis; a mis-sense mutation in rll4 attenuates its suppressing function, leading to a high concentration of anthocyanins. Sequence analysis of the RLL1-RLL4 genes from wild and cultivated lettuce showed that their function-changing mutations occurred after domestication. The mutations in rll1 disrupt anthocyanin biosynthesis, while the mutations in RLL2, rll3 and rll4 activate anthocyanin biosynthesis, showing disruptive selection for leaf colour during domestication of lettuce. The characterization of multiple polymorphic genes in this study provides the necessary molecular resources for the rational breeding of lettuce cultivars with distinct levels of red pigments and green cultivars with high levels of health-promoting flavonoids.

SUBMITTER: Su W

PROVIDER: S-EPMC6953203 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:Light and its spectral characteristics are crucial for plant growth and development. The far-red photon flux mediates many plant processes through the action of phytochrome and also accelerates the photosynthetic electron transfer rate. In this study, we assessed the effects of far-red addition on butterhead lettuce morphology, light use efficiency, optical properties, and phytochemical characteristics. Three-week-old lettuce plants (Lactuca sativa L. cv. Alyssa) were grown for up to 28 days under a 10% blue and 90% red light spectrum (200 µmol m-2 s-1, 16 h photoperiod) to which five different intensities of far-red light (peak at 735 nm) were added (0-9-18-36-72 µmol m-2 s-1). White light-emitting diodes were included as a proxy for sunlight. Increasing supplemental far-red photon flux from zero to 21% increased the light use efficiency (g per mol) by 37% on day 14; 43% on day 21; and 39% on day 28. Measurements of projected head area suggest that this was associated with an increase in leaf expansion and photon capture and not necessarily a direct effect on photosynthesis. Moreover, vegetation indices based on leaf reflectance showed a decrease in chlorophyll-related indices under a high far-red photon flux. This decrease in pigment content was confirmed by chemical analyses, suggesting that the plants may not reach their full potential in terms of photon capture, limiting the overall photosynthetic performance. Furthermore, the stress-related Carter 1 index increased in plants grown under a high far-red photon flux, indicating early plant stress. Far-red tended to decrease the content of total phenolics and increase soluble sugars. The higher sugar levels can be attributed to an improved photochemical efficiency due to photosystem I excitation by far-red wavelengths, also known as the Emerson Enhancement effect. Despite these higher sugar levels, no effect on foliar nitrate content was observed. Our results show that far-red supplementation has the potential to enhance light interception at the early growth stages, although higher intensities of far-red may cause plant stress.

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Characterization of four polymorphic genes controlling red leaf colour in lettuce that have undergone disruptive selection since domestication.

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