Ontology highlight
ABSTRACT:
SUBMITTER: Du Z
PROVIDER: S-EPMC6959005 | biostudies-literature | 2019 Oct
REPOSITORIES: biostudies-literature
Du Ziming Z Lin Jia-Ren JR Rashid Rumana R Maliga Zoltan Z Wang Shu S Aster Jon C JC Izar Benjamin B Sorger Peter K PK Santagata Sandro S
Nature protocols 20190918 10
Multiplexed tissue imaging enables precise, spatially resolved enumeration and characterization of cell types and states in human resection specimens. A growing number of methods applicable to formalin-fixed, paraffin-embedded (FFPE) tissue sections have been described, the majority of which rely on antibodies for antigen detection and mapping. This protocol provides step-by-step procedures for confirming the selectivity and specificity of antibodies used in fluorescence-based tissue imaging and ...[more]