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A Comparison Study on the Characteristics of Nanofibrils Isolated from Fibers and Parenchyma Cells in Bamboo.

ABSTRACT: In this study, bamboo fibers and parenchyma cells were separated by a physical water-medium method. To compare the characteristics of nanofibrils from these two types of cells, lignocellulose nanofibrils (LCNFs) and cellulose nanofibrils (CNFs) were prepared by different processes. Atomic force microscopy analysis revealed that both fibers and parenchyma cells can be separated into individual fibrils after grinding three times. However, LCNFs had a diameter of 20-40 nm, which was larger than that of CNFs (10-20 nm). Additionally, the films prepared from LCNFs had lower tensile strength, but higher hydrophobicity compared with those from CNFs. X-ray diffraction analysis and tensile test of the films showed that the nanofibrils isolated from fibers and parenchyma cells had similar crystallinity and mechanical properties. This study shows a promising application of bamboo parenchyma cells, which are usually discarded as waste in the processing of bamboo products, in the preparation of nanofibers.

SUBMITTER: Zhang X

PROVIDER: S-EPMC6981400 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature

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A Comparison Study on the Characteristics of Nanofibrils Isolated from Fibers and Parenchyma Cells in Bamboo.

Zhang Xiaofeng X Huang Hanxiao H Qing Yan Y Wang Hankun H Li Xingong X

Materials (Basel, Switzerland) 20200106 1

In this study, bamboo fibers and parenchyma cells were separated by a physical water-medium method. To compare the characteristics of nanofibrils from these two types of cells, lignocellulose nanofibrils (LCNFs) and cellulose nanofibrils (CNFs) were prepared by different processes. Atomic force microscopy analysis revealed that both fibers and parenchyma cells can be separated into individual fibrils after grinding three times. However, LCNFs had a diameter of 20-40 nm, which was larger than tha ...[more]

PMID: 31935802

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Project description:The paraveinal mesophyll (PVM) of soybean leaves is a layer of laterally expanded cells sandwiched between the palisade and spongy mesophyll chlorenchyma. The vacuoles of PVM cells contain an abundance of a putative vegetative storage protein, VSP (α, β). VSP is is constitutively produced, but is up-regulated during sink limitation experiments involving flower, fruit, or vegetative bud removal. Soybean vegetative lipoxygenases (Vlx), consisting of 5 isozymes (Vlx, A-D), have been identified as potential storage proteins because they accumulate to high levels with experimental sink limitation and have been co-localized with VSP to the vacuoles of PVM cells. We re-investigated the sub-cellular locations of these enzymes with TEM immuno-cytochemistry. We employed laser micro-dissection to compared RNA expression of PVM cells with mesophyll chlorenchyma cells; and we performed a micro-array analysis of soybean leaf samples representing a time-course, sink-limitation, experiment. We found that none of the Vlx isozymes co-localize with putative storage proteins in PVM vacuoles, and that our sink limitation experiment (typical of those used in the past) induced a strong up-regulation of stress response genes, simultaneous with the up-regulation of the Vlx isozymes. Our findings do not support a storage function for soybean Vlx. The microarray results presented here represent our comparison of gene expression within PVM and palisade mesophyll paraenchyma (PP) cells from mature soybean leaves. For this part of our study both the PVM and palisade parenchyma (PP) cells were isolated from sections of soybean leaves by laser micro-dissection and pressure catapulting (LMPC). Total RNA was isolated from these cells and amplified for microarray analysis. Soybean leaves were fixed with a mixture of ethanol and acetic acid, embedded in paraffin and sectioned to provide samples for laser micro-dissection with pressure catapulting (LMPC). Slides were deparaffinized prior to LMPC. A total of six samples were collected: three biological replicates for PVM cells and three replicates for PP cells. After enough cells were collected for each samples, the cells were pooled for RNA extraction. RNA was amplified prior to microarray hybridization using the Whole-Transcriptome Ovation Pico RNA Amplification System from NuGEN Technologies, San Carlos, CA, USA.

Dataset Information

A Comparison Study on the Characteristics of Nanofibrils Isolated from Fibers and Parenchyma Cells in Bamboo.

Publications

A Comparison Study on the Characteristics of Nanofibrils Isolated from Fibers and Parenchyma Cells in Bamboo.

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