Dataset Information

Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues.

ABSTRACT:

Purpose

Herein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation.

Methods

Magnetic resonance imaging was performed on room-temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T₁ mapping and T2∗ -weighted images were acquired using sweep imaging with Fourier transformation and gradient-echo sequences, respectively. Verification of IONP localization was performed through histological assessment and microcomputer tomography.

Results

Quantitative imaging was achieved for organs and VCAs perfused with up to 642 mM_Fe (36 mg_Fe /mL), which is above previous demonstrations of upper limit detection in agarose (35.7mM_Fe [2 mg_Fe /mL]). The stability of IONPs in the perfusate had an effect on the quality of distribution and imaging within organs or VCA. Finally, MRI provided more accurate IONP localization than Prussian blue histological staining in this system, wherein IONPs remain primarily in the vasculature.

Conclusion

Using MRI, we were able to assess the distribution of IONPs throughout organs and VCAs varying in complexity. Additional studies are necessary to better understand this system and validate the calibration between T₁ measurements and IONP concentration.

SUBMITTER: Ring HL

PROVIDER: S-EPMC6982566 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Publications

Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues.

Ring Hattie L HL Gao Zhe Z Sharma Anirudh A Han Zonghu Z Lee Charles C Brockbank Kelvin G M KGM Greene Elizabeth D ED Helke Kristi L KL Chen Zhen Z Campbell Lia H LH Weegman Bradley B Davis Monica M Taylor Michael M Giwa Sebastian S Fahy Gregory M GM Wowk Brian B Pagotan Roberto R Bischof John C JC Garwood Michael M

Magnetic resonance in medicine 20191209 5

<h4>Purpose</h4>Herein, we evaluate the use of MRI as a tool for assessing iron oxide nanoparticle (IONP) distribution within IONP perfused organs and vascularized composite allografts (VCAs) (i.e., hindlimbs) prepared for cryopreservation.<h4>Methods</h4>Magnetic resonance imaging was performed on room-temperature organs and VCAs perfused with IONPs and were assessed at 9.4 T. Quantitative T<sub>1</sub> mapping and T2∗ -weighted images were acquired using sweep imaging with Fourier transformati ...[more]

PMID: 31815324

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Project description:PurposeTo determine the feasibility of T2-weighted magnetic resonance (MR) imaging in the noninvasive quantification of renal inflammation by using superparamagnetic iron oxide (SPIO) nanoparticles targeted to tissue-bound C3 activation fragments in a mouse model of lupus nephritis.Materials and methodsAll animal procedures were approved by the University of Colorado-Denver animal care and use committee. SPIO nanoparticles were encapsulated by using amine-functionalized phospholipids. A recombinant protein containing the C3d-binding region of complement receptor type 2 (CR2) was then conjugated to the surface of the SPIO nanoparticle. Five MRL/lpr mice (a model of lupus nephritis) and six C57BL/6 wild-type mice were assessed with T2-weighted MR imaging at baseline and after SPIO injection. The same five MRL/lpr mice and three C57BL/6 mice also underwent MR imaging after injection of CR2-targeted SPIO. A series of T2-weighted pulses with 16 echo times was used to enable precise T2 mapping and calculation of T2 relaxation times in the cortex and outer and inner medulla of the kidneys, as well as in the spleen, muscle, and fat. The effects of treatment and animal genotype on T2 relaxation times were analyzed with repeated-measures analysis of variance.ResultsAt baseline, the T2-weighted signal intensity in the kidneys of MRL/lpr mice was higher than that in the kidneys of wild-type mice. Injection of untargeted SPIO did not alter the T2-weighted signal in the kidneys in either strain of mice. Injection of CR2-targeted SPIO in MRL/lpr mice, however, caused a significant accumulation of targeted iron oxide with a subsequent decrease in T2 relaxation times in the cortex and outer and inner medulla of the kidneys. No changes in T2 relaxation time were observed in the wild-type mice after injection of targeted SPIO.ConclusionInjection of CR2-conjugated SPIO caused a significant reduction in T2-weighted MR imaging signal and T2 relaxation time in nephritic kidneys.

Dataset Information

Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues.

Purpose

Methods

Results

Conclusion

Publications

Imaging the distribution of iron oxide nanoparticles in hypothermic perfused tissues.

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