Ontology highlight
ABSTRACT:
SUBMITTER: Hasegawa Y
PROVIDER: S-EPMC6994043 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Hasegawa Yoshinori Y Ikeno Masashi M Suzuki Nobutaka N Nakayama Manabu M Ohara Osamu O
Biology methods & protocols 20181231 1
A human artificial chromosome (HAC) vector has potential to overcome the problems of stable gene expression associated with plasmid, transposon, and virus-based vectors, such as insertional mutagenesis, position effect, uncontrollable copy number, unstable gene expression, and DNA size limitation. The main advantages of the HAC are its episomal nature and ability to accommodate DNA inserts of any size. However, HAC vectors have two disadvantages: low efficiency of gene insertion and lack of repo ...[more]