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Bioconversion of glycerol into lipids by Rhodosporidium toruloides in a two-stage process and characterization of lipid properties.

ABSTRACT: Rhodosporidium toruloides AS 2.1389 has been considered a promising oleaginous strain due to its flexible substrate adaptability, high lipid content, and coproduction of some pigments. In previous work, R. toruloides has shown good potential to directly convert crude glycerol into intracellular lipids. However, the difference in nutritional demand between cell growth and lipid accumulation was found to be a dilemma. Therefore, a glycerol-based two-stage process was proposed in the present work to separately meet the nutritional demand of both the cell proliferation phase and lipid accumulation phase. Factors that affect microbial conversion of glycerol into lipid were investigated, statistically analyzed, and optimized. As a result, 26.5 g L-1 biomass with 10 g L-1 lipid was obtained in the two-stage process. Lipid yield (0.20 g g-1) and productivity (0.083 g L-1 h-1) achieved were significantly higher than the previously optimized batch culture. In R. toruloides lipids, the dominant fatty acid compositions are palmitic acid (28.5%), stearic acid (12.9%), oleic acid (41.3%), and linoleic acid (12.8%). Phospholipids accounts for 0.63% in total lipid. Lipase-catalyzed methanolysis could achieve up to 95% biodiesel yield. The characterization of R. toruloides lipid suggests its great application potential for biodiesel and specialty-type lipid products.

SUBMITTER: Xu J

PROVIDER: S-EPMC6999356 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Bioconversion of glycerol into lipids by Rhodosporidium toruloides in a two-stage process and characterization of lipid properties.

Xu Jingyang J Zhao Xuebing X Du Wei W Liu Dehua D

Engineering in life sciences 20160816 3

Rhodosporidium toruloides AS 2.1389 has been considered a promising oleaginous strain due to its flexible substrate adaptability, high lipid content, and coproduction of some pigments. In previous work, R. toruloides has shown good potential to directly convert crude glycerol into intracellular lipids. However, the difference in nutritional demand between cell growth and lipid accumulation was found to be a dilemma. Therefore, a glycerol-based two-stage process was proposed in the ...[more]

PMID: 32624776

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Project description:Background:Lipid accumulation by oleaginous microorganisms is of great scientific interest and biotechnological potential. While nitrogen limitation has been routinely employed, low-cost raw materials usually contain rich nitrogenous components, thus preventing from efficient lipid production. Inorganic phosphate (Pi) limitation has been found sufficient to promote conversion of sugars into lipids, yet the molecular basis of cellular response to Pi limitation and concurrent lipid accumulation remains elusive. Results:Here, we performed multi-omic analyses of the oleaginous yeast Rhodosporidium toruloides to shield lights on Pi-limitation-induced lipid accumulation. Samples were prepared under Pi-limited as well as Pi-repleted chemostat conditions, and subjected to analysis at the transcriptomic, proteomic, and metabolomic levels. In total, 7970 genes, 4212 proteins, and 123 metabolites were identified. Results showed that Pi limitation facilitates up-regulation of Pi-associated metabolism, RNA degradation, and triacylglycerol biosynthesis while down-regulation of ribosome biosynthesis and tricarboxylic acid cycle. Pi limitation leads to dephosphorylation of adenosine monophosphate and the allosteric activator of isocitrate dehydrogenase key to lipid biosynthesis. It was found that NADPH, the key cofactor for fatty acid biosynthesis, is limited due to reduced flux through the pentose phosphate pathway and transhydrogenation cycle and that this can be overcome by over-expression of an endogenous malic enzyme. These phenomena are found distinctive from those under nitrogen limitation. Conclusions:Our data suggest that Pi limitation activates Pi-related metabolism, RNA degradation, and TAG biosynthesis while inhibits ribosome biosynthesis and TCA cycle, leading to enhanced carbon fluxes into lipids. The information greatly enriches our understanding on microbial oleaginicity and Pi-related metabolism. Importantly, systems data may facilitate designing advanced cell factories for production of lipids and related oleochemicals.

Dataset Information

Bioconversion of glycerol into lipids by Rhodosporidium toruloides in a two-stage process and characterization of lipid properties.

Publications

Bioconversion of glycerol into lipids by <i>Rhodosporidium toruloides</i> in a two-stage process and characterization of lipid properties.

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