Dataset Information

Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum.

ABSTRACT: Abiotic and biotic stresses widely reduce light harvesting complex (LHC) gene expression in higher plants and algae. However, control mechanisms and functions of these changes are not well understood. During herbivory, marine diatom species release oxylipins that impair grazer reproduction and serve as signaling molecules to nearby undamaged diatoms. To examine LHC mRNA regulation by oxylipin exposure, the diatom Phaeodactylum tricornutum was treated with a sublethal concentration of trans,trans-2,4-decadienal (DD) during the light cycle. Transcriptome analyses revealed extensive suppression of LHC mRNAs and a smaller set of up-regulated LHC mRNAs at 3?h. For two divergently regulated LHCF antennae family mRNAs, in vivo 4-thiouracil metabolic labeling was used to distinguish synthesis and degradation rates. Within 3?h of DD exposure, Lhcf2 mRNA levels and transcription were strongly suppressed and its mRNA half-life decreased. In contrast, Lhcf15 mRNA mainly accumulated between 3-9?h, its transcription increased and its mRNA was highly stabilized. Hence, DD-treated cells utilized transcriptional and mRNA stability control mechanisms which were likely major factors in the differing Lhcf2 and Lhcf15 expression patterns. Widespread LHC mRNA regulation and possible effects on photosynthesis may contribute to enhanced fitness in cells impacted by herbivory and other stresses.

SUBMITTER: Islam S

PROVIDER: S-EPMC7005025 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum.

Islam Shahima S Sabharwal Tanya T Wu Samantha S Bullock T J TJ Mehdy Mona C MC

Scientific reports 20200206 1

Abiotic and biotic stresses widely reduce light harvesting complex (LHC) gene expression in higher plants and algae. However, control mechanisms and functions of these changes are not well understood. During herbivory, marine diatom species release oxylipins that impair grazer reproduction and serve as signaling molecules to nearby undamaged diatoms. To examine LHC mRNA regulation by oxylipin exposure, the diatom Phaeodactylum tricornutum was treated with a sublethal concentration of trans,trans ...[more]

PMID: 32029835

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Project description:Photosynthetic diatoms that live suspended throughout the water column will constantly be swept up and down by vertical mixing. When returned to the photic zone after experiencing longer periods in darkness, mechanisms exist that enable the diatoms both to survive sudden light exposure and immediately utilize the available energy in photosynthesis and growth. We have investigated both the response to prolonged darkness and the re-acclimation to moderate intensity white irradiance (E?=?100 µmol m(-2) s(-1)) in the diatom Phaeodactylum tricornutum, using an integrated approach involving global transcriptional profiling, pigment analyses, imaging and photo-physiological measurements. The responses were studied during continuous white light, after 48 h of dark treatment and after 0.5 h, 6 h, and 24 h of re-exposure to the initial irradiance. The analyses resulted in several intriguing findings. Dark treatment of the cells led to 1) significantly decreased nuclear transcriptional activity, 2) distinct intracellular changes, 3) fixed ratios of the light-harvesting pigments despite a decrease in the total cell pigment pool, and 4) only a minor drop in photosynthetic efficiency (?(PSII_max)). Re-introduction of the cells to the initial light conditions revealed 5) distinct expression profiles for nuclear genes involved in photosynthesis and those involved in photoprotection, 6) rapid rise in photosynthetic parameters (? and rETR(max)) within 0.5 h of re-exposure to light despite a very modest de novo synthesis of photosynthetic compounds, and 7) increasingly efficient resonance energy transfer from fucoxanthin chlorophyll a/c-binding protein complexes to photosystem II reaction centers during the first 0.5 h, supporting the observations stated in 6). In summary, the results show that despite extensive transcriptional, metabolic and intracellular changes, the ability of cells to perform photosynthesis was kept intact during the length of the experiment. We conclude that P. tricornutum maintains a functional photosynthetic apparatus during dark periods that enables prompt recovery upon re-illumination.

Dataset Information

Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum.

Publications

Early dynamics of photosynthetic Lhcf2 and Lhcf15 transcription and mRNA stabilities in response to herbivory-related decadienal in Phaeodactylum tricornutum.

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