Ontology highlight
ABSTRACT:
SUBMITTER: Liu C
PROVIDER: S-EPMC7021225 | biostudies-literature | 2019 Aug
REPOSITORIES: biostudies-literature
Liu Chunxiao C Barrett Taylor M TM Chen Xing X Ferrie John J JJ Petersson E James EJ
Chembiochem : a European journal of chemical biology 20190614 16
Thioamide substitutions of the peptide backbone have been shown to reduce proteolytic degradation, and this property can be used to generate competitive protease inhibitors and to stabilize peptides toward degradation in vivo. Here, we present a straightforward sensor design that allows a systematic study of the positional effects of thioamide substitution by using real-time fluorescence. Thioamide scanning in peptide substrates of five papain family cysteine proteases demonstrates that a thioam ...[more]