Ontology highlight
ABSTRACT:
SUBMITTER: Teplova M
PROVIDER: S-EPMC7027511 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Teplova Marianna M Falschlunger Christoph C Krasheninina Olga O Egger Michaela M Ren Aiming A Patel Dinshaw J DJ Micura Ronald R
Angewandte Chemie (International ed. in English) 20200109 7
Pistol ribozymes constitute a new class of small self-cleaving RNAs. Crystal structures have been solved, providing three-dimensional snapshots along the reaction coordinate of pistol phosphodiester cleavage, corresponding to the pre-catalytic state, a vanadate mimic of the transition state, and the product. The results led to the proposed underlying chemical mechanism. Importantly, a hydrated Mg<sup>2+</sup> ion remains innersphere-coordinated to N7 of G33 in all three states, and is consistent ...[more]