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Prediction and identification of novel HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from endocan.


ABSTRACT: Background:Prediction and identification of cytotoxic T lymphocyte (CTL) epitopes from tumor associated antigens is a crucial step for the development of tumor immunotherapy strategy. Endocan has been identified as antigen overexpressed in various tumors. Methods:In this experiment, we predicted and identified HLA-A2-restricted CTL epitopes from endocan by using the following procedures. Firstly, we predicted the epitopes from the amino acid sequence of endocan by computer-based methods; Secondly, we determined the affinity of the predicted peptide with HLA-A2.1 molecule by peptide-binding assay; Thirdly, we elicited the primary T cell response against the predicted peptides in vitro; Lastly, we tested the specific CTLs toward endocan and HLA-A2.1 positive target cells. Results:These data demonstrated that peptides of endocan containing residues 4-12 and 9-17 could elicit specific CTLs producing interferon-? and cytotoxicity. Conclusions:Therefore, our findings suggested that the predicted peptides were novel HLA-A2.1-restricted CTL epitopes, and might provide promising target for tumor immunotherapy.

SUBMITTER: Shao G 

PROVIDER: S-EPMC7031931 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Prediction and identification of novel HLA-A*0201-restricted cytotoxic T lymphocyte epitopes from endocan.

Shao Gaohai G   Liu Qingjun Q   Yang Ling L   Feng Guibo G   Zhao Wang W   Huang Zhongyan Z   Yang Zhao Z  

Journal of inflammation (London, England) 20200219


<h4>Background</h4>Prediction and identification of cytotoxic T lymphocyte (CTL) epitopes from tumor associated antigens is a crucial step for the development of tumor immunotherapy strategy. Endocan has been identified as antigen overexpressed in various tumors.<h4>Methods</h4>In this experiment, we predicted and identified HLA-A2-restricted CTL epitopes from endocan by using the following procedures. Firstly, we predicted the epitopes from the amino acid sequence of endocan by computer-based m  ...[more]

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