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Inorganic Sol-Gel Polymerization for Hydrogel Bioprinting.

ABSTRACT: An inorganic sol-gel polymerization process was used as a cross-linking reaction during three-dimensional (3D) bioprinting of cell-containing hydrogel scaffolds. Hybrid hydroxypropyl methyl cellulose (HPMC), with a controlled ratio of silylation, was prepared and isolated as a 3D-network precursor. When dissolved in a biological buffer containing human mesenchymal stem cells, it yields a bioink that can be printed during polymerization by extrusion. It is worth noting that the sol-gel process proceeded at pH 7.4 using biocompatible mode of catalysis (NaF and glycine). The printing window was determined by rheology and viscosity measurements. The physicochemical properties of hydrogels were studied. Covalent functionalization of the network can be easily performed by adding a triethoxysilyl-containing molecule; a fluorescent hybrid molecule was used as a proof of concept.

SUBMITTER: Montheil T

PROVIDER: S-EPMC7033675 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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Inorganic Sol-Gel Polymerization for Hydrogel Bioprinting.

Montheil Titouan T Maumus Marie M Valot Laurine L Lebrun Aurélien A Martinez Jean J Amblard Muriel M Noël Danièle D Mehdi Ahmad A Subra Gilles G

ACS omega 20200206 6

An inorganic sol-gel polymerization process was used as a cross-linking reaction during three-dimensional (3D) bioprinting of cell-containing hydrogel scaffolds. Hybrid hydroxypropyl methyl cellulose (HPMC), with a controlled ratio of silylation, was prepared and isolated as a 3D-network precursor. When dissolved in a biological buffer containing human mesenchymal stem cells, it yields a bioink that can be printed during polymerization by extrusion. It is worth noting that the sol-gel process pr ...[more]

PMID: 32095687

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