Dataset Information

R1? sensitivity to pH and other compounds at clinically accessible spin-lock fields in the presence of proteins.

ABSTRACT: Numerous human diseases involve abnormal metabolism, and proton exchange is an effective source of magnetic resonance imaging (MRI) contrast for assessing metabolism. One MRI technique that capitalizes on proton exchange is R₁ relaxation in the rotating frame (R_1? ). Here, we investigated the sensitivity of R_1? to various proton-exchange mechanisms at spin-lock pulses within Food and Drug Administration (FDA) safety guidelines for radiofrequency-induced heating. We systematically varied pH known to change the rate of proton exchange as well as the glucose and lysine concentrations, thus changing the number of amide, hydroxyl and amine exchangeable sites in a series of egg-white albumin phantoms. The resulting effects on quantitative relaxation time measurements of R_1? , R₁ and R₂ were observed at 3 T. Using spin-lock amplitudes available for human imaging (less than 23.5 ?T) at near physiologic temperatures, we found R_1? was more sensitive to physiologic changes in pH than to changes in glucose and lysine concentrations. In addition, R_1? was more sensitive to pH changes than R₁ and R₂ . Models of proton exchange fitted to the relaxation measurements suggest that amide groups were the primary source of pH sensitivity. Together, these experiments suggest an optimal spin-lock amplitude for measuring pH changes while not exceeding FDA-subject heating limitations.

SUBMITTER: Owusu N

PROVIDER: S-EPMC7043777 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

R1ρ sensitivity to pH and other compounds at clinically accessible spin-lock fields in the presence of proteins.

Owusu Nana N Johnson Casey P CP Kearney William W Thedens Dan D Wemmie John J Magnotta Vincent A VA

NMR in biomedicine 20191119 2

Numerous human diseases involve abnormal metabolism, and proton exchange is an effective source of magnetic resonance imaging (MRI) contrast for assessing metabolism. One MRI technique that capitalizes on proton exchange is R1 relaxation in the rotating frame (R1ρ ). Here, we investigated the sensitivity of R1ρ to various proton-exchange mechanisms at spin-lock pulses within Food and Drug Administration (FDA) safety guidelines for radiofrequency-induced heating. ...[more]

PMID: 31742802

Similar Datasets

Project description:BackgroundR1ρ (or spin-lock) imaging is prone to artifacts arising from field inhomogeneities that may impact the R1ρ quantification. Previous research has proposed two types of method to manage the artifacts in continuous-wave constant amplitude spin-lock, one is based on the composite block pulses to compensate for the field imperfections, another category uses adiabatic pulses in the R1ρ pre-pulse to excite and reverse the magnetization (named adiabatic prepared approach). Although both methods have proved their efficiency in alleviating artifacts, we observed that the adiabatic pulse approach could produce much lower R1ρ dispersion in human knee cartilage than the block pulse method (characterized by the R1ρ difference ∆R1ρ =11.4 Hz (from spin-lock field 50 to 500 Hz) for the block pulse method vs. ∆R1ρ =4.5 Hz for the adiabatic pulse approach). Prompted by this observation, the purpose of this study was to investigate the underlying factors that may affect the R1ρ dispersion through numerical simulations based on the two-pool exchanging Bloch-McConnell equations.MethodsThe effects of free water pool size Pa (from 0.80 to 0.95), chemical exchange rate kb (from the bound to free water pool, ranged from 500 to 3,000 Hz), adiabatic pulse duration Tp (from 5.0 to 25 ms), and the chemical shift of the bound pool ppmb (from 1.0 to 5.0 ppm) were examined on the degree of the R1ρ dispersion for the two R1ρ imaging methods.ResultsIn general, the greater the ppmb, kb, Tp, and the smaller Pa, the more significant difference in R1ρ dispersion between the block and adiabatic approaches, with the dispersion curve of the adiabatic method becoming flatter.ConclusionsThe adiabatic prepared approach may compromise the R1ρ dispersion, the effect is determined by the combination of the tissue and radiofrequency (RF) pulse properties. It is suggested that care should be taken when using the adiabatically prepared approach to study R1ρ dispersion.

Dataset Information

R1? sensitivity to pH and other compounds at clinically accessible spin-lock fields in the presence of proteins.

Publications

R1ρ sensitivity to pH and other compounds at clinically accessible spin-lock fields in the presence of proteins.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets