Ontology highlight
ABSTRACT:
SUBMITTER: Ustinova K
PROVIDER: S-EPMC7049964 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Ustinova Kseniya K Novakova Zora Z Saito Makoto M Meleshin Marat M Mikesova Jana J Kutil Zsofia Z Baranova Petra P Havlinova Barbora B Schutkowski Mike M Matthias Patrick P Barinka Cyril C
The Journal of biological chemistry 20200117 9
Histone deacetylase 6 (HDAC6) is a multidomain cytosolic enzyme having tubulin deacetylase activity that has been unequivocally assigned to the second of the tandem catalytic domains. However, virtually no information exists on the contribution of other HDAC6 domains on tubulin recognition. Here, using recombinant protein expression, site-directed mutagenesis, fluorimetric and biochemical assays, microscale thermophoresis, and total internal reflection fluorescence microscopy, we identified the ...[more]