Dataset Information
Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure.
Ontology highlight
ABSTRACT: Purpose
To assess testicular mRNA and protein expression levels of MRE11 and RAD50 in human azoospermia patients.Methods
Patients diagnosed with maturation arrest at the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) were recruited through diagnostic testicular biopsy. Patients with normal spermatogenesis were studied as controls. In addition, knockdown of MRE11 and RAD50 was performed in GC-2spd(ts) cells to investigate their roles in cellular proliferation and apoptosis.Results
mRNA and protein expression levels of MRE11 and RAD50 were measured using quantitative polymerase chain reaction, western blotting, and immunohistochemistry, respectively. Knockdown of both MRE11 and RAD50 utilized transfection with small interfering RNAs.Conclusion
Our findings demonstrated altered expression levels of MRE11 and RAD50 in human testes with MA and SCOS, and showed that these alterations might be associated with impaired spermatogenesis. These results offer valuable new perspectives into the molecular mechanisms of male infertility.
SUBMITTER: Hu M
PROVIDER: S-EPMC7056783 | biostudies-literature | 2020 Feb
REPOSITORIES: biostudies-literature
Publications
Decreased expression of MRE11 and RAD50 in testes from humans with spermatogenic failure.
Journal of assisted reproduction and genetics 20200125 2
<h4>Purpose</h4>To assess testicular mRNA and protein expression levels of MRE11 and RAD50 in human azoospermia patients.<h4>Methods</h4>Patients diagnosed with maturation arrest at the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) were recruited through diagnostic testicular biopsy. Patients with normal spermatogenesis were studied as controls. In addition, knockdown of MRE11 and RAD50 was performed in GC-2spd(ts) cells to investigate their roles in cellular proliferation and ap ...[more]
