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The use of local structural similarity of distant homologues for crystallographic model building from a molecular-replacement solution.


ABSTRACT: The performance of automated protein model building usually decreases with resolution, mainly owing to the lower information content of the experimental data. This calls for a more elaborate use of the available structural information about macromolecules. Here, a new method is presented that uses structural homologues to improve the quality of protein models automatically constructed using ARP/wARP. The method uses local structural similarity between deposited models and the model being built, and results in longer main-chain fragments that in turn can be more reliably docked to the protein sequence. The application of the homology-based model extension method to the example of a CFA synthase at 2.7?Å resolution resulted in a more complete model with almost all of the residues correctly built and docked to the sequence. The method was also evaluated on 1493 molecular-replacement solutions at a resolution of 4.0?Å and better that were submitted to the ARP/wARP web service for model building. A significant improvement in the completeness and sequence coverage of the built models has been observed.

SUBMITTER: Chojnowski G 

PROVIDER: S-EPMC7057216 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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The use of local structural similarity of distant homologues for crystallographic model building from a molecular-replacement solution.

Chojnowski Grzegorz G   Choudhury Koushik K   Heuser Philipp P   Sobolev Egor E   Pereira Joana J   Oezugurel Umut U   Lamzin Victor S VS  

Acta crystallographica. Section D, Structural biology 20200228 Pt 3


The performance of automated protein model building usually decreases with resolution, mainly owing to the lower information content of the experimental data. This calls for a more elaborate use of the available structural information about macromolecules. Here, a new method is presented that uses structural homologues to improve the quality of protein models automatically constructed using ARP/wARP. The method uses local structural similarity between deposited models and the model being built,  ...[more]

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