Dataset Information

A Near-infrared Turn-on Fluorescent Sensor for Sensitive and Specific Detection of Albumin from Urine Samples.

ABSTRACT: A readily synthesizable fluorescent probe DMAT-?-CAP was evaluated for sensitive and selective detection of human serum albumin (HSA). DMAT-?-CAP showed selective turn-on fluorescence at 730 nm in the presence of HSA with more than 720-fold enhancement in emission intensity ([DMAT-?-CAP] = 10 ?M), and rapid detection of HSA was accomplished in 3 seconds. The fluorescence intensity of DMAT-?-CAP was shown to increase in HSA concentration-dependent manner (Kd = 15.4 ± 3.3 ?M), and the limit of detection of DMAT-?-CAP was determined to be 10.9 nM (0.72 mg/L). The 1:1 stoichiometry between DMAT-?-CAP and HSA was determined, and the displacement assay revealed that DMAT-?-CAP competes with hemin for the unique binding site, which rarely accommodates drugs and endogenous compounds. Based on the HSA-selective turn-on NIR fluorescence property as well as the unique binding site, DMAT-?-CAP was anticipated to serve as a fluorescence sensor for quantitative detection of the HSA level in biological samples with minimized background interference. Thus, urine samples were directly analyzed by DMAT-?-CAP to assess albumin levels, and the results were comparable to those obtained from immunoassay. The similar sensitivity and specificity to the immunoassay along with the simple, cost-effective, and fast detection of HSA warrants practical application of the NIR fluorescent albumin sensor, DMAT-?-CAP, in the analysis of albumin levels in various biological environments.

SUBMITTER: Kim Y

PROVIDER: S-EPMC7070906 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature

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A Near-infrared Turn-on Fluorescent Sensor for Sensitive and Specific Detection of Albumin from Urine Samples.

Kim Yoonjeong Y Shin Eunryeol E Jung Woong W Kim Mi Kyoung MK Chong Youhoon Y

Sensors (Basel, Switzerland) 20200224 4

A readily synthesizable fluorescent probe DMAT-π-CAP was evaluated for sensitive and selective detection of human serum albumin (HSA). DMAT-π-CAP showed selective turn-on fluorescence at 730 nm in the presence of HSA with more than 720-fold enhancement in emission intensity ([DMAT-π-CAP] = 10 μM), and rapid detection of HSA was accomplished in 3 seconds. The fluorescence intensity of DMAT-π-CAP was shown to increase in HSA concentration-dependent manner (<i>K</i><sub>d</sub> = 15.4 ± 3.3 μM), an ...[more]

PMID: 32102360

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Dataset Information

A Near-infrared Turn-on Fluorescent Sensor for Sensitive and Specific Detection of Albumin from Urine Samples.

Publications

A Near-infrared Turn-on Fluorescent Sensor for Sensitive and Specific Detection of Albumin from Urine Samples.

Similar Datasets

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