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Molecular characterization of a canine coronavirus NA/09 strain detected in a dog's organs.

ABSTRACT: In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is characteristic of CB/05, indicates the need to identify new genetic markers for pantropic variants of CCoV, probably in the spike-protein gene region.

SUBMITTER: Ntafis V

PROVIDER: S-EPMC7087105 | biostudies-literature | 2012 Jan

REPOSITORIES: biostudies-literature

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Molecular characterization of a canine coronavirus NA/09 strain detected in a dog's organs.

Ntafis Vasileios V Xylouri Eftychia E Mari Viviana V Papanastassopoulou Maria M Papaioannou Nikolaos N Thomas Angelos A Buonavoglia Canio C Decaro Nicola N

Archives of virology 20111016 1

In the present study, the detection of a pantropic canine coronavirus (CCoV) strain in a dog with lethal diarrhoea is reported. RT-PCR and real-time RT-PCR assays were used for the detection, characterization and quantitation of CCoV. Sequence and phylogenetic analysis of the CCoV NA/09 revealed a high degree of sequence identity with the pantropic strain CB/05, indicating the presence of CB/05-like pantropic strains in Greece. The absence of the 38-nucleotide deletion in ORF3b, which is charact ...[more]

PMID: 22002680

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