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Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase.


ABSTRACT: Long-chain alk(a/e)nes represent the major constituents of conventional transportation fuels. Biosynthesis of alkanes is ubiquitous in many kinds of organisms. Cyanobacteria possess two enzymes, acyl-acyl carrier protein (acyl-ACP) reductase (AAR) and aldehyde-deformylating oxygenase (ADO), which function in a two-step alkane biosynthesis pathway. These two enzymes act in series and possibly form a complex that efficiently converts long chain fatty acyl-ACP/fatty acyl-CoA into hydrocarbon. While the structure of ADO has been previously described, structures of both AAR and AAR-ADO complex have not been solved, preventing deeper understanding of this pathway. Here, we report a ligand-free AAR structure, and three AAR-ADO complex structures in which AARs bind various ligands. Our results reveal the binding pattern of AAR with its substrate/cofactor, and suggest a potential aldehyde-transferring channel from AAR to ADO. Based on our structural and biochemical data, we proposed a model for the complete catalytic cycle of AAR.

SUBMITTER: Gao Y 

PROVIDER: S-EPMC7089970 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Structural insights into catalytic mechanism and product delivery of cyanobacterial acyl-acyl carrier protein reductase.

Gao Yu Y   Zhang Hongmei H   Fan Minrui M   Jia Chenjun C   Shi Lifang L   Pan Xiaowei X   Cao Peng P   Zhao Xuelin X   Chang Wenrui W   Li Mei M  

Nature communications 20200323 1


Long-chain alk(a/e)nes represent the major constituents of conventional transportation fuels. Biosynthesis of alkanes is ubiquitous in many kinds of organisms. Cyanobacteria possess two enzymes, acyl-acyl carrier protein (acyl-ACP) reductase (AAR) and aldehyde-deformylating oxygenase (ADO), which function in a two-step alkane biosynthesis pathway. These two enzymes act in series and possibly form a complex that efficiently converts long chain fatty acyl-ACP/fatty acyl-CoA into hydrocarbon. While  ...[more]

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