Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana.
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ABSTRACT:
SUBMITTER: Takaoka Y
PROVIDER: S-EPMC7093514 | biostudies-literature | 2020 Mar
REPOSITORIES: biostudies-literature
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Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana.
Takaoka Yousuke Y Miyagawa Saki S Nakamura Akinobu A Egoshi Syusuke S Tsukiji Shinya S Ueda Minoru M
Scientific reports 20200324 1
In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc<sub>2</sub>Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple ...[more]
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