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Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins.

ABSTRACT: Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the quantification of 67% of all murine proteins annotated by UniProtKB/Swiss-Prot. Furthermore, we applied the spectral library to SWATH-MS data from murine tissue samples. Data are available via SWATHAtlas (PASS01441).

SUBMITTER: Zhong CQ

PROVIDER: S-EPMC7099061 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Generation of a murine SWATH-MS spectral library to quantify more than 11,000 proteins.

Zhong Chuan-Qi CQ Wu Jianfeng J Qiu Xingfeng X Chen Xi X Xie Changchuan C Han Jiahuai J

Scientific data 20200326 1

Targeted SWATH-MS data analysis is critically dependent on the spectral library. Comprehensive spectral libraries of human or several other organisms have been published, but the extensive spectral library for mouse, a widely used model organism is not available. Here, we present a large murine spectral library covering more than 11,000 proteins and 240,000 proteotypic peptides, which included proteins derived from 9 murine tissue samples and one murine L929 cell line. This resource supports the ...[more]

PMID: 32218446

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