A comprehensive spectral assay library to quantify the Escherichia coli proteome by DIA/SWATH-MS.

ABSTRACT: Data-Independent Acquisition (DIA) is a method to improve consistent identification and precise quantitation of peptides and proteins by mass spectrometry (MS). The targeted data analysis strategy in DIA relies on spectral assay libraries that are generally derived from a priori measurements of peptides for each species. Although Escherichia coli (E. coli) is among the best studied model organisms, so far there is no spectral assay library for the bacterium publicly available. Here, we generated a spectral assay library for 4,014 of the 4,389 annotated E. coli proteins using one- and two-dimensional fractionated samples, and ion mobility separation enabling deep proteome coverage. We demonstrate the utility of this high-quality library with robustness in quantitation of the E. coli proteom

SUBMITTER: Midha MK 

PROVIDER: S-EPMC7665006 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature