Innate Activation of IFN-?-iNOS Axis During Infection With Salmonella Represses the Ability of T Cells to Produce IL-2.

ABSTRACT: Pathogenic Salmonella serovars are a major cause of enteric illness in humans and animals, and produce clinical manifestations ranging from localized gastroenteritis to systemic disease. T cells are a critical component of immunity against this intracellular pathogen. The mechanisms by which Salmonella modulates T-cell-mediated immune responses in order to establish systemic infection are not completely understood. We show that infection of mice with Salmonella enterica serovar Typhimurium (S. Typhimurium) suppresses IL-2 and increases IFN-? and IL-17 production from T cells activated in vivo or ex vivo through the T cell receptor. Infection with S. Typhimurium brings about recruitment of CD11b+Gr1+ suppressor cells to the spleen. Ex vivo depletion of these cells restores the ability of activated T cells to produce IL-2 and brings secretion of IFN-? and IL-17 from these cells back to basal levels. The reduction in IL-2 secretion is not seen in IFN-?-/- and iNOS-/- mice infected with Salmonella. Our findings demonstrate that sustained innate activated IFN-? production during progression of infection with Salmonella reduces IL-2-secreting capability of T cells through an iNOS-mediated signaling pathway that can adversely affect long term immunity against this pathogen.

SUBMITTER: Yadav J 

PROVIDER: S-EPMC7109407 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature