Foot and mouth disease virus undergoes non-progressive replication in mice peritoneal macrophages and induces M1 polarization.

ABSTRACT: Despite the fact that macrophages link the innate and adaptive arms of immunity, it's role in the early infection of foot and mouth disease virus (FMDV) is largely unknown. Recently, depletion of macrophages in vivo after vaccination has shown to drastically diminish the protection against FMDV challenge in mouse model. Even the ability of macrophages to reduce or resist FMDV infection is not known hitherto. Therefore, we examined the replication ability of FMDV in mice peritoneal macrophages and the responsiveness in terms of macrophage polarization and cytokine production. Negative strand specific RT-PCR indicated replication of FMDV RNA in macrophages. Absolute quantitation of FMDV transcripts, immunofluorescence studies and titre of the infectious progeny virus revealed that replicatio

SUBMITTER: Sebastian R 

PROVIDER: S-EPMC7114663 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature