Ontology highlight
ABSTRACT:
SUBMITTER: Chen R
PROVIDER: S-EPMC7119747 | biostudies-literature | 2004 Dec
REPOSITORIES: biostudies-literature
Chen Ru R Huang Weiming W Lin Zhixiong Z Zhou Zhongfang Z Yu Haiqiong H Zhu Daozhong D
Journal of virological methods 20041201 1
Real-time RT-PCR assay, based on light upon extension (LUX) fluorogenic primer and LightCycle technology, was developed for rapid detection of transmissible gastroenteritis virus (TGEV). Viral RNA from different TGEV isolates and clinical specimens was detected. To evaluate the sensitivity of the assay, a gel-based RT-PCR method targeted at the same 101 bp sequence was also developed. Serial 10-fold dilutions of TGEV RNA were detected by the two methods. Although the real time method used only 2 ...[more]