Ontology highlight
ABSTRACT:
SUBMITTER: Cardoso TC
PROVIDER: S-EPMC7125592 | biostudies-literature | 2010 Dec
REPOSITORIES: biostudies-literature
Molecular and cellular probes 20100821 6
A sensitive reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the rapid visual detection of turkey coronavirus (TCoV) infection. The reaction is performed in one step in a single tube at 65 °C for 45 min, with hydroxynaphthol blue (HNB) dye added prior to amplification. The detection limit of the RT-LAMP assay was approximately 10(2) EID(50/50 μl) TCoV genome, and no cross-reaction with other avian viruses was observed. The assay was evaluated further ...[more]