Ontology highlight
ABSTRACT:
SUBMITTER: Kim S
PROVIDER: S-EPMC7132813 | biostudies-literature | 2020 Apr
REPOSITORIES: biostudies-literature
Kim Soojeong S Darcy Isabel K IK
Biology open 20200403 4
An experimental technique called difference topology combined with the mathematics of tangle analysis has been used to unveil the structure of DNA bound by the Mu transpososome. However, difference topology experiments can be difficult and time consuming. We discuss a modification that greatly simplifies this experimental technique. This simple experiment involves using a topoisomerase to trap DNA crossings bound by a protein complex and then running a gel to determine the crossing number of the ...[more]