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Chemical Characteristics and Anticancer Activity of Essential Oil from Arnica Montana L. Rhizomes and Roots.

ABSTRACT: Arnica montana L. is a medicinal plant with diverse biological activities commonly used in pharmacy and cosmetics. The attributes of A. montana are mainly related to the concentration and chemical composition of essential oils (EOs). Therefore, the objective of this study was to characterize the chemical composition of EOs derived from A. montana rhizomes and roots taking into account the age of the plants and to investigate the effect of the analyzed EOs on induction of apoptosis, necrosis, and autophagy in human glioblastoma multiforme T98G and anaplastic astrocytoma MOGGCCM cell lines. Rhizomes and roots of mountain arnica were harvested at the end of the third and fourth vegetation periods. The chemical composition of essential oils was determined with the GC-MS technique. Among the 37 components of the essential oil of A. montana, 2,5-dimethoxy-p-cymene (46.47%-60.31%), 2,6-diisopropylanisole (14.48%-23.10%), thymol methyl ether (5.31%-17.79%), p-methoxyheptanophenone (5.07%-9.65%), and ?-isocomene (0.68%-2.87%), were detected in the rhizomes and roots of the three-year-old plants and in the rhizomes and roots of the four-year-old plants. The plant part (rhizome, root) and plant age can be determinants of the essential oil composition and, consequently, their biological activity. The induction of apoptosis (but not autophagy nor necrosis) at a level of 28.5%-32.3% is a promising result, for which 2,5-dimethoxy-p-cymene, 2,6-diisopropylanisole, thymol methyl ether, and p-methoxyheptanophenone are probably mainly responsible. The present study is the first report on the anticancer activities of essential oils from A. montana rhizomes and roots.

SUBMITTER: Sugier P

PROVIDER: S-EPMC7143959 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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Chemical Characteristics and Anticancer Activity of Essential Oil from Arnica Montana L. Rhizomes and Roots.

Sugier Piotr P Jakubowicz-Gil Joanna J Sugier Danuta D Kowalski Radosław R Gawlik-Dziki Urszula U Kołodziej Barbara B Dziki Dariusz D

Molecules (Basel, Switzerland) 20200312 6

Arnica montana L. is a medicinal plant with diverse biological activities commonly used in pharmacy and cosmetics. The attributes of A. montana are mainly related to the concentration and chemical composition of essential oils (EOs). Therefore, the objective of this study was to characterize the chemical composition of EOs derived from A. montana rhizomes and roots taking into account the age of the plants and to investigate the effect of the analyzed EOs on induction of apo ...[more]

PMID: 32178275

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Project description:BackgroundConventional cancer treatments, such as chemotherapy, radiation therapy, and surgery, often affect the patients' quality of life due to their serious side effects, indicating the urgent need to develop less toxic and more effective alternative treatments. Medicinal plants and their derivatives are invaluable sources for such remedies. The present study aimed to determine the chemical composition, anticancer and antibacterial activities of Nepeta mahanesis essential oil (EO).MethodsThe chemical composition of EO was analyzed by gas chromatography-mass spectrometry (GC-MS). Cytotoxicity and apoptosis/necrosis induction of EO was analyzed by MTT assay and Flow cytometry. Real-time PCR was performed to evaluate the Bax/Bcl2 gene expression. Also, the effect of the EO on the cells' mitochondrial membrane potential (MMP) and ROS level was assessed. DPPH assay was done to assess the free radical scavenging activity of the EO. The Antimicrobial activity, MIC, and MBC of the oil were determined via well-diffusion and broth microdilution methods.ResultsBased on the GC-MS analysis, 24 compounds were identified in the EO, of which 1,8-cineole (28.5%), Nepetalactone (18.8%), germacrene D (8.1%), and β-pinene (7.2%), were the major compounds. Also, the EO showed considerable cytotoxicity against MCF-7, Caco-2, SH-SY5Y, and HepG2 after 24 and 48 h treatment with IC50 values between 0.0.47 to 0.81 mg/mL. It was revealed that this compound increased the Bax/Bcl2 ratio in the MCF-7 cells and induced apoptosis (27%) and necrosis (18%) in the cells. Moreover, the EO treatment led to a substantial decrease in MMP, which is indicative of apoptosis induction. A significant increase in ROS level was also detected in the cells following exposure to the EO. This compound showed strong DPPH radical scavenging activity (IC50: 30). It was also effective against Gram-positive E. faecalis (ATCC 29,212) and Gram-negative E. coli (ATCC 11,333) bacteria.ConclusionsThe results of this study demonstrated that the EO of N. mahanesis could be considered a bioactive product with biomedical applications that can be used as an alternative cancer treatment and applied in the biomedical industries.

Project description:Damask rose (Rosa damascena Mill.) is an aromatic medicinal plant rich in bioactive compounds with high value in the food, pharmaceutical and cosmetic industries. Knowledge of the factors affecting the quantitative and qualitative properties of the compounds in its essential oil (EO) and the bioactivity of this EO is important in optimizing Damask rose cultivation and production. This research studied, for the first time, the effects of irrigation water and soil chemical and physical characteristics on the EO yield of this important commercial species and on it chemical composition and antimicrobial activity. The results showed the significant effect of crop cultivation site on yield, chemical composition and inhibition zone diameter (IZD) at the 1% significance level. The highest EO yield (~0.0266%), which belonged to the Noushabad site (EON), resulted from the increased soil electrical conductivity (EC) and the higher sand, gypsum and lime contents and irrigation water salinity. Analysis of the chemical composition of the EOs showed that their main compounds at all three crop sites were citronellol, nonadecane, heneicosane and geraniol. The EO obtained from the Yazdel site (EOY) had the highest contents of citronellol and geraniol (~29.05% and ~6.85%) that were directly correlated with soil potassium and phosphorus contents and inversely correlated with soil acidity and EC and its lime, nitrogen, and organic carbon contents. Antimicrobial assays indicated that the EO extracted from the Sefidshahr site (EOS), which had the largest inhibition zone diameter (~14.67 mm) for Aspergillus brasiliensis (IZD~14.67 mm) and the lowest MIC (~31.25 μg/mL) for Staphylococcus aureus and Pseudomonas aeruginosa, exhibited efficacy similar to that of rifampin, probably due to the dominance of the alkanes in it. The EOY and EOS also exhibited the strongest inhibitory and lethal activity against Candida albicans (MIC and MBC <15.63 μg/mL for EOY and MIC and MBC = 62.5 μg/mL for EOS), which were six and four times stronger than those of nystatin, respectively. Therefore, the selected EOs can act as a potentially promising strategy for fighting microbial strains.

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Chemical Characteristics and Anticancer Activity of Essential Oil from Arnica Montana L. Rhizomes and Roots.

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Chemical Characteristics and Anticancer Activity of Essential Oil from <i>Arnica Montana</i> L. Rhizomes and Roots.

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