Ontology highlight
ABSTRACT:
SUBMITTER: Neef AB
PROVIDER: S-EPMC7159598 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Neef Anne B AB Pernot Lucile L Schreier Verena N VN Scapozza Leonardo L Luedtke Nathan W NW
Angewandte Chemie (International ed. in English) 20150514 27
Pathogen-selective labeling was achieved by using the novel gemcitabine metabolite analogue 2'-deoxy-2',2'-difluoro-5-ethynyluridine (dF-EdU) and click chemistry. Cells infected with Herpes Simplex Virus-1 (HSV-1), but not uninfected cells, exhibit nuclear staining upon the addition of dF-EdU and a fluorescent azide. The incorporation of the dF-EdU into DNA depends on its phosphorylation by a herpes virus thymidine kinase (TK). Crystallographic analyses revealed how dF-EdU is well accommodated i ...[more]