Genomics

Dataset Information

0

Bioorthogonal Chemical Epigenetic Modifiers Enable Dose-dependent CRISPR Targeted Gene Activation


ABSTRACT: CRISPR-Cas9 systems have been developed to regulate gene expression by using either fusions to epigenetic regulators or, more recently, through the use of chemically mediated strategies. These approaches have armed researchers with new tools to examine the function of proteins by intricately controlling expression levels of specific genes. Here, we present a CRISPR based chemical approach that uses a new Chemical Epigenetic Modifier (CEM) to hone to a gene targeted with a catalytically inactive Cas9 (dCas9) bridged to an FK506-binding protein (FKBP). One arm of the bifunctional CEM recruits BRD4 to the target site and the other arm is composed of a bumped ligand that binds to a mutant FKBP with a compensatory hole at F36V. This bump-and-hole strategy allows for activation of target genes in a dose dependent and reversible fashion with increased specificity and high efficacy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE185912 | GEO | 2022/08/22

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA419663 | ENA
2019-04-30 | GSE129407 | GEO
2016-08-20 | E-GEOD-72259 | biostudies-arrayexpress
2023-06-16 | E-MTAB-13009 | biostudies-arrayexpress
2016-07-29 | E-GEOD-84534 | biostudies-arrayexpress
2014-06-18 | E-GEOD-58511 | biostudies-arrayexpress
2015-08-10 | E-GEOD-71430 | biostudies-arrayexpress
2021-09-30 | E-MTAB-10914 | biostudies-arrayexpress
2024-07-10 | E-MTAB-13503 | biostudies-arrayexpress
2022-03-05 | E-MTAB-11470 | biostudies-arrayexpress