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Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO₂ nanofiber filter.

ABSTRACT: There is an urgent need to develop an automated malaria diagnostic system that can easily and rapidly detect malaria parasites and determine the proportion of malaria-infected erythrocytes in the clinical blood samples. In this study, we developed a quantitative, mobile, and fully automated malaria diagnostic system equipped with an on-disc SiO₂ nanofiber filter and blue-ray devices. The filter removes the leukocytes and platelets from the blood samples, which interfere with the accurate detection of malaria by the blue-ray devices. We confirmed that the filter, which can be operated automatically by centrifugal force due to the rotation of the disc, achieved a high removal rate of leukocytes (99.7%) and platelets (90.2%) in just 30?s. The automated system exhibited a higher sensitivity (100%) and specificity (92.8%) for detecting Plasmodium falciparum from the blood of 274 asymptomatic individuals in Kenya when compared to the common rapid diagnosis test (sensitivity?=?98.1% and specificity = 54.8%). This indicated that this system can be a potential alternative to conventional methods used at local health facilities, which lack basic infrastructure.

SUBMITTER: Yamamoto T

PROVIDER: S-EPMC7171072 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO<sub>2</sub> nanofiber filter.

Yamamoto Takeki T Hashimoto Muneaki M Nagatomi Kenji K Nogami Takahiro T Sofue Yasuyuki Y Hayashi Takuya T Ido Yusuke Y Yatsushiro Shouki S Abe Kaori K Kajimoto Kazuaki K Tamari Noriko N Awuor Beatrice B Sonye George G Kongere James J Munga Stephen S Ohashi Jun J Oka Hiroaki H Minakawa Noboru N Kataoka Masatoshi M Mita Toshihiro T

Scientific reports 20200420 1

There is an urgent need to develop an automated malaria diagnostic system that can easily and rapidly detect malaria parasites and determine the proportion of malaria-infected erythrocytes in the clinical blood samples. In this study, we developed a quantitative, mobile, and fully automated malaria diagnostic system equipped with an on-disc SiO<sub>2</sub> nanofiber filter and blue-ray devices. The filter removes the leukocytes and platelets from the blood samples, which interfere with the accur ...[more]

PMID: 32313065

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Project description:SiO2@TiO2 core-shell nanoparticles were successfully synthesized via a simple, reproducible, and low-cost method and tested for methylene blue adsorption and UV photodegradation, with a view to their application in wastewater treatment. The monodisperse SiO2 core was obtained by the classical Stöber method and then coated with a thin layer of TiO2, followed by calcination or hydrothermal treatments. The properties of SiO2@TiO2 core-shell NPs resulted from the synergy between the photocatalytic properties of TiO2 and the adsorptive properties of SiO2. The synthesized NPs were characterized using FT-IR spectroscopy, HR-TEM, FE-SEM, and EDS. Zeta potential, specific surface area, and porosity were also determined. The results show that the synthesized SiO2@TiO2 NPs that are hydrothermally treated have similar behaviors and properties regardless of the hydrothermal treatment type and synthesis scale and better performance compared to the SiO2@TiO2 calcined and TiO2 reference samples. The generation of reactive species was determined by EPR, and the photocatalytic activity was evaluated by the methylene blue (MB) removal in aqueous solution under UV light. Hydrothermally treated SiO2@TiO2 showed the highest adsorption capacity and photocatalytic removal of almost 100% of MB after 15 min in UV light, 55 and 89% higher compared to SiO2 and TiO2 reference samples, respectively, while the SiO2@TiO2 calcined sample showed 80%. It was also observed that the SiO2-containing samples showed a considerable adsorption capacity compared to the TiO2 reference sample, which improved the MB removal. These results demonstrate the efficient synergy effect between SiO2 and TiO2, which enhances both the adsorption and photocatalytic properties of the nanomaterial. A possible photocatalytic mechanism was also proposed. Also noteworthy is that the performance of the upscaled HT1 sample was similar to one of the lab-scale synthesized samples, demonstrating the potentiality of this synthesis methodology in producing candidate nanomaterials for the removal of contaminants from wastewater.

Dataset Information

Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO₂ nanofiber filter.

Publications

Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO<sub>2</sub> nanofiber filter.

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OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO2 nanofiber filter.

Publications

Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO<sub>2</sub> nanofiber filter.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Development of a quantitative, portable, and automated fluorescent blue-ray device-based malaria diagnostic equipment with an on-disc SiO₂ nanofiber filter.