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Expanding the genetic code of the human hematopoietic system.

ABSTRACT: The genetic incorporation of noncanonical amino acids (ncAAs) into proteins has been realized in bacteria, yeast, and mammalian cells, and recently, in multicellular organisms including plants and animals. However, the addition of new building blocks to the genetic code of tissues from human origin has not yet been achieved. To this end, we report a self-replicating Epstein-Barr virus-based episomal vector for the long-term encoding of ncAAs in human hematopoietic stem cells and reconstitution of this genetically engineered hematopoietic system in mice.

SUBMITTER: Shao S

PROVIDER: S-EPMC7183198 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Expanding the genetic code of the human hematopoietic system.

Shao Sida S Koh Minseob M Schultz Peter G PG

Proceedings of the National Academy of Sciences of the United States of America 20200406 16

The genetic incorporation of noncanonical amino acids (ncAAs) into proteins has been realized in bacteria, yeast, and mammalian cells, and recently, in multicellular organisms including plants and animals. However, the addition of new building blocks to the genetic code of tissues from human origin has not yet been achieved. To this end, we report a self-replicating Epstein-Barr virus-based episomal vector for the long-term encoding of ncAAs in human hematopoietic stem cells and reconstitution o ...[more]

PMID: 32253306

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