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Exploratory analysis of age and sex dependent DNA methylation patterns on the X-chromosome in whole blood samples.

ABSTRACT: BACKGROUND:Large numbers of autosomal sites are found differentially methylated in the aging genome. Due to analytical difficulties in dealing with sex differences in X-chromosome content and X-inactivation (XCI) in females, this has not been explored for the X chromosome. METHODS:Using data from middle age to elderly individuals (age 55+?years) from two Danish cohorts of monozygotic twins and the Scottish Lothian Birth Cohort 1921, we conducted an X-chromosome-wide analysis of age-associated DNA methylation patterns with consideration of stably inferred XCI status. RESULTS:Through analysing and comparing sex-specific X-linked DNA methylation changes over age late in life, we identified 123, 293 and 55 CpG sites significant (FDR?

SUBMITTER: Li S

PROVIDER: S-EPMC7189689 | biostudies-literature | 2020 Apr

REPOSITORIES: biostudies-literature

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Exploratory analysis of age and sex dependent DNA methylation patterns on the X-chromosome in whole blood samples.

Li Shuxia S Lund Jesper B JB Christensen Kaare K Baumbach Jan J Mengel-From Jonas J Kruse Torben T Li Weilong W Mohammadnejad Afsaneh A Pattie Alison A Marioni Riccardo E RE Deary Ian J IJ Tan Qihua Q

Genome medicine 20200428 1

<h4>Background</h4>Large numbers of autosomal sites are found differentially methylated in the aging genome. Due to analytical difficulties in dealing with sex differences in X-chromosome content and X-inactivation (XCI) in females, this has not been explored for the X chromosome.<h4>Methods</h4>Using data from middle age to elderly individuals (age 55+ years) from two Danish cohorts of monozygotic twins and the Scottish Lothian Birth Cohort 1921, we conducted an X-chromosome-wide analysis of ag ...[more]

PMID: 32345361

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