Unknown

Dataset Information

0

Degradation of Herpes Simplex Virus-1 Viral miRNA H11 by Vaccinia Virus Protein VP55 Attenuates Viral Replication.


ABSTRACT: Among 29 distinct miRNAs expressed by the herpes simplex virus-1 (HSV-1) during lytic infection, miR-H11, together with miR-H1 to miR-H8 are reported to locate in the RNA-induced silencing complex (RISC). miR-H11 is encoded within viral origins of replication and lies entirely within the origins of replication. However, the roles of this miRNA derived from lytic infection with HSV-1 remain unclear. Using the advantage of vaccinia virus protein VP55 (VP55)-mediated degradation of miRNAs, we constructed a recombinant virus expressing VP55 (R5502) to demonstrate that: (1) accumulation of miR-H11 from R5502 was reduced by 540-fold versus that in cells infected with wild-type HSV-1, but miR-H1 to miR-H8 which also located in the RISC were not reduced significantly from R5502 compare with wild-type HSV-1; (2) downregulation of miR-H11 from R5502 infected cells results in markedly lower viral DNA synthesis compared with wild-type HSV-1; and (3) downregulation of miR-H11 also restricted viral spreading, and resulted in low accumulation of representative viral proteins and viral yields. The findings were confirmed through either using of a miR-H11 inhibitor or pre-transfection of a plasmid expressing VP55. These data suggest that miR-H11 plays a currently unidentified role in maintaining sufficient viral DNA synthesis during the course of viral infection.

SUBMITTER: Zou W 

PROVIDER: S-EPMC7191008 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

altmetric image

Publications

Degradation of Herpes Simplex Virus-1 Viral miRNA H11 by Vaccinia Virus Protein VP55 Attenuates Viral Replication.

Zou Weixuan W   Zhou Xusha X   Wang Lei L   Zhou Grace Guoying GG   Chen Xiaoqing X  

Frontiers in microbiology 20200423


Among 29 distinct miRNAs expressed by the herpes simplex virus-1 (HSV-1) during lytic infection, miR-H11, together with miR-H1 to miR-H8 are reported to locate in the RNA-induced silencing complex (RISC). miR-H11 is encoded within viral origins of replication and lies entirely within the origins of replication. However, the roles of this miRNA derived from lytic infection with HSV-1 remain unclear. Using the advantage of vaccinia virus protein VP55 (VP55)-mediated degradation of miRNAs, we const  ...[more]

Similar Datasets

| S-EPMC5309293 | biostudies-literature
2008-04-03 | E-GEOD-10962 | biostudies-arrayexpress
| S-EPMC6094942 | biostudies-literature
| S-EPMC8389370 | biostudies-literature
| S-EPMC5520797 | biostudies-literature
| S-EPMC6338833 | biostudies-literature
| S-EPMC8735597 | biostudies-literature
| S-EPMC7016767 | biostudies-literature
| S-EPMC2603433 | biostudies-literature
2008-04-03 | GSE10962 | GEO