Dataset Information

Comparison of the accuracy of different impression procedures in case of multiple and angulated implants : Accuracy of impressions in multiple and angulated implants.

ABSTRACT: BACKGROUND:There is no consensus in the literature regarding the impression procedures in the presence of multiple and angulated implants. METHODS:Three maxillary master models with 6 implants bilaterally positioned in anterior, premolar and molar regions were fabricated. In model 1, all implants were placed in parallel; in models 2 and 3, anterior implants were buccally inclined and posterior implants were distally inclined in 10- and 20-degrees, respectively. Three different impression copings (hexed, non-hex, multi-unit) and two different impression techniques (splinting and non-splinting) were tested. A total of 180 impressions (n?=?10 per group) were made using mono-phase vinyl poly-siloxane. Master models and duplicate casts were scanned by a 5-axis laboratory scanner and data were transferred to a software program for the alignment of master and duplicate copings. Coronal and angular deviations were calculated, and data were statistically analyzed. RESULTS:For angulated models, the lowest deviation values were detected at the splinted non-hex coping group (P?

SUBMITTER: Richi MW

PROVIDER: S-EPMC7197148 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Comparison of the accuracy of different impression procedures in case of multiple and angulated implants : Accuracy of impressions in multiple and angulated implants.

Richi M Wafa MW Kurtulmus-Yilmaz Sevcan S Ozan Oguz O

Head & face medicine 20200504 1

<h4>Background</h4>There is no consensus in the literature regarding the impression procedures in the presence of multiple and angulated implants.<h4>Methods</h4>Three maxillary master models with 6 implants bilaterally positioned in anterior, premolar and molar regions were fabricated. In model 1, all implants were placed in parallel; in models 2 and 3, anterior implants were buccally inclined and posterior implants were distally inclined in 10- and 20-degrees, respectively. Three different imp ...[more]

PMID: 32366261

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Project description:The advancement of intraoral scanners has allowed for more efficient workflow in the dental clinical setting. However, limited data exist regarding the accuracy of the digital impressions produced with various scanner settings and scanning approaches. The purpose of this in vitro study was to compare the accuracy of digital impressions at the crown preparation margin using different scanning resolutions of a specific intraoral scanner system. An all-ceramic crown preparation of a mandibular first molar was constructed in a typodont, and a scan (n = 3) was created with an industrial-grade laboratory scanner (3Shape D2000) as the control. Digital impressions were obtained with an intraoral scanner (3Shape TRIOS 3) under three settings-high resolution (HR), standard resolution (SR), and combined resolution (SHR). Comparative 3D analysis of scans was performed with Geomagic Control X software to measure the discrepancy between intraoral scans and the control scan along the preparation finish line. The scan time and number of images captured per scan were recorded. Statistical analysis was performed by one-way ANOVA, two-way repeated measures ANOVA, Pearson's correlation, and Dunnett's T3 test (? = 0.05). Significant differences were observed for scan time and for number of images captured among scan resolution settings (? < 0.05). The scan time for the SR group was, on average, 34.2 s less than the SHR group and 46.5 s less than the HR group. For discrepancy on the finish line, no significant differences were observed among scanning resolutions (HR: 31.5 ± 5.5 ?m, SHR: 33.2 ± 3.7 ?m, SR: 33.6 ± 3.1 ?m). Significant differences in discrepancy were observed among tooth surfaces, with the distal surface showing the highest discrepancies. In conclusion, the resolution of the intra-oral scanner is primarily defined by the system hardware and optimized for default scans. A software high-resolution mode that obtains more data over a longer time may not necessarily benefit the scan accuracy, while the tooth preparation and surface parameters do affect the accuracy.

Project description:Synaptosomes are frequently used research objects in neurobiology studies focusing on synaptic transmission as they mimic several aspects of the physiological synaptic functions. They contain the whole apparatus for neurotransmission, the presynaptic nerve ending with synaptic vesicles, synaptic mitochondria and often a segment of the postsynaptic membrane along with the postsynaptic density is attached to its outer surface. As being artificial functional organelles, synaptosomes are viable for several hours, retain their activity, membrane potential, and capable to store, release, and reuptake neurotransmitters. Synaptosomes are ideal subjects for proteomic analysis. The recently available separation and protein detection techniques can cope with the reduced complexity of the organelle and enable the simultaneous qualitative and quantitative analysis of thousands of proteins shaping the structural and functional characteristics of the synapse. Synaptosomes are formed during the homogenization of nervous tissue in the isoosmotic milieu and can be isolated from the homogenate by various approaches. Each enrichment method has its own benefits and drawbacks and there is not a single method that is optimal for all research purposes. For a proper proteomic experiment, it is desirable to preserve the native synaptic structure during the isolation procedure and keep the degree of contamination from other organelles or cell types as low as possible. In this article, we examined five synaptosome isolation methods from a proteomic point of view by the means of electron microscopy, Western blot, and liquid chromatography-mass spectrometry to compare their efficiency in the isolation of synaptosomes and depletion of contaminating subcellular structures. In our study, the different isolation procedures led to a largely overlapping pool of proteins with a fairly similar distribution of presynaptic, active zone, synaptic vesicle, and postsynaptic proteins; however, discrete differences were noticeable in individual postsynaptic proteins and in the number of identified transmembrane proteins. Much pronounced variance was observed in the degree of contamination with mitochondrial and glial structures. Therefore, we suggest that in selecting the appropriate isolation method for any neuroproteomics experiment carried out on synaptosomes, the degree and sort/source of contamination should be considered as a primary aspect.

Dataset Information

Comparison of the accuracy of different impression procedures in case of multiple and angulated implants : Accuracy of impressions in multiple and angulated implants.

Publications

Comparison of the accuracy of different impression procedures in case of multiple and angulated implants : Accuracy of impressions in multiple and angulated implants.

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