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Method for the synthesis of flavonoid nitrogen mustard derivatives.

ABSTRACT: We have prepared a kind of new type of flavonoid nitrogen mustard derivatives with broad-spectrum antitumor activity, which have not been reported in the literature. In the process of preparing intermediate (the flavonoid diethanolamine derivatives) of the target compound, we found that the reasonable separation method is silica gel column chromatography with eluent (MeOH/CH2Cl2, 1:40). The experimental results show that the composition of eluent is an important factor to get high yield of the intermediate, which will directly affect the final yield of the target compound. Acetonitrile is the suitable solvent in the reaction system, and the optimized reaction condition is reaction under reflux condition for 48 hours. Several new flavonoid nitrogen mustard derivatives were synthesized with high yield using the above method.•A method for the synthesis of flavonoid nitrogen mustard derivatives was introduced.•The reasonable purification conditions and the optimized reaction time were recommended.

SUBMITTER: Song J

PROVIDER: S-EPMC7210452 | biostudies-literature | 2020

REPOSITORIES: biostudies-literature

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Method for the synthesis of flavonoid nitrogen mustard derivatives.

Song Jinglei J Yu Meixuan M Yan Xi X Hao Haijun H

MethodsX 20200425

We have prepared a kind of new type of flavonoid nitrogen mustard derivatives with broad-spectrum antitumor activity, which have not been reported in the literature. In the process of preparing intermediate (the flavonoid diethanolamine derivatives) of the target compound, we found that the reasonable separation method is silica gel column chromatography with eluent (MeOH/CH<sub>2</sub>Cl<sub>2</sub>, 1:40). The experimental results show that the composition of eluent is an important factor to g ...[more]

PMID: 32405467

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Project description:Sulfur mustard (SM) is a potent vesicant that targets epithelial cells and tissues. Most vesicant research has been performed using bona fide SM; however, some studies have used simulants, most notably half mustard (2-chloroethyl ethylsulfide; CEES) and nitrogen mustard (mechlorethamine; NM). Although CEES and NM have similarities to SM and can cause vesication, there are distinct differences in the chemical structures and physical properties of these compounds that may impact their toxic effects. Microarray analysis of cultured primary human epidermal keratinocytes (HEK) exposed to each of these vesicants was performed to directly compare the transcriptional responses induced by these vesicants. HEK were exposed in triplicate to concentrations ranging from 0-1000 µM for SM and NM and 0-4000 µM for CEES. Cells were harvested at 1, 2, 4, 8, 16, and 24 h and the RNA isolated for microarray analysis. Transcriptional responses were phenotypically anchored to cell morphology. The dataset was filtered by exposure and timepoint, and an analysis of variance was performed using dose as the factor. The top 500 genes ranked by p-value were analyzed using gene ontology algorithms to identify biological pathways significantly affected by each vesicant. At 2 h post-exposure, p53 signaling, Erk/MAPK signaling, and BMP signaling were significantly affected by all three vesicants. At 4 h post-exposure, p53 signaling , B cell activating factor, and glucocorticoid receptor signaling were significantly affected by all three vesicants. At 8 h post-exposure, there were no significant pathways commonly affected by all three vesicants. These results suggest that, although there are similarities in the transcriptional responses to each of these vesicants, the transcriptional responses appear to differ over time. Thus, extrapolation of results obtained with one vesicant to other vesicants may be complex and may have important implications for the development of vesicant therapeutics.

Dataset Information

Method for the synthesis of flavonoid nitrogen mustard derivatives.

Publications

Method for the synthesis of flavonoid nitrogen mustard derivatives.

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