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DNA 5-Methylcytosine-Specific Amplification and Sequencing.

ABSTRACT: DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy.

SUBMITTER: Liu C

PROVIDER: S-EPMC7216556 | biostudies-literature | 2020 Mar

REPOSITORIES: biostudies-literature

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DNA 5-Methylcytosine-Specific Amplification and Sequencing.

Liu Chang C Cui Xiaolong X Zhao Boxuan Simen BS Narkhede Pradnya P Gao Yawei Y Liu Jun J Dou Xiaoyang X Dai Qing Q Zhang Li-Sheng LS He Chuan C

Journal of the American Chemical Society 20200225 10

DNA 5-methylcytosine (5mC)-specific mapping has been hampered by severe DNA degradation and the presence of 5-hydroxymethylcytosine (5hmC) using the conventional bisulfite sequencing approach. Here, we present a 5mC-specific whole-genome amplification method (5mC-WGA), with which we achieved 5mC retention during DNA amplification from limited input down to 10 pg scale with limited interference from 5hmC signals, providing DNA 5mC methylome with high reproducibility and accuracy. ...[more]

PMID: 32077696

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