Dynamic changes in circulating T follicular helper cell composition predict neutralising antibody responses after yellow fever vaccination.

ABSTRACT:

Objectives

T follicular helper (Tfh) cells are the principal T helper cell subset that provides help to B cells for potent antibody responses against various pathogens. In this study, we took advantage of the live-attenuated yellow fever virus (YFV) vaccine strain, YF-17D, as a model system for studying human antiviral immune responses in vivo following exposure to an acute primary virus challenge under safe and highly controlled conditions, to comprehensively analyse the dynamics of circulating Tfh (cTfh) cells.

Methods

We tracked and analysed the response of cTfh and other T and B cell subsets in peripheral blood of healthy volunteers by flow cytometry over the course of 4 weeks after YF-17D vaccination.

Results

Using surface staining of cell activation marke

SUBMITTER: Huber JE 

PROVIDER: S-EPMC7221214 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature