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Super-Resolution Reconstruction for Two- and Three-Dimensional LA-ICP-MS Bioimaging.


ABSTRACT: The resolution of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) elemental bioimaging is usually constrained by the diameter of the laser spot size and is often not adequate to explore in situ subcellular distributions of elements and proteins in biological tissue sections. Super-resolution reconstruction is a method typically used for many imaging modalities and combines multiple lower resolution images to create a higher resolution image. Here, we present a super-resolution reconstruction method for LA-ICP-MS imaging by ablating consecutive layers of a biological specimen with offset orthogonal scans, resulting in a 10× improvement in resolution for quantitative measurement of dystrophin in murine muscle fibers. Layer-by-layer image reconstruction was also extended to the third dimension without the requirement of image registration across multiple thin section specimens. Quantitative super-resolution reconstruction, combined with Gaussian filtering and application of the Richardson-Lucy total variation algorithm, provided superior image clarity and fidelity in two- and three-dimensions.

SUBMITTER: Westerhausen MT 

PROVIDER: S-EPMC7232986 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature

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Super-Resolution Reconstruction for Two- and Three-Dimensional LA-ICP-MS Bioimaging.

Westerhausen Mika T MT   Bishop David P DP   Dowd Annette A   Wanagat Jonathan J   Cole Nerida N   Doble Philip A PA  

Analytical chemistry 20191107 23


The resolution of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) elemental bioimaging is usually constrained by the diameter of the laser spot size and is often not adequate to explore in situ subcellular distributions of elements and proteins in biological tissue sections. Super-resolution reconstruction is a method typically used for many imaging modalities and combines multiple lower resolution images to create a higher resolution image. Here, we present a super-resol  ...[more]

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