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Structural Biology in the Multi-Omics Era.

ABSTRACT: Rapid developments in cryogenic electron microscopy have opened new avenues to probe the structures of protein assemblies in their near native states. Recent studies have begun applying single -particle analysis to heterogeneous mixtures, revealing the potential of structural-omics approaches that combine the power of mass spectrometry and electron microscopy. Here we highlight advances and challenges in sample preparation, data processing, and molecular modeling for handling increasingly complex mixtures. Such advances will help structural-omics methods extend to cellular-level models of structural biology.

SUBMITTER: McCafferty CL

PROVIDER: S-EPMC7254829 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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Structural Biology in the Multi-Omics Era.

McCafferty Caitlyn L CL Verbeke Eric J EJ Marcotte Edward M EM Taylor David W DW

Journal of chemical information and modeling 20200310 5

Rapid developments in cryogenic electron microscopy have opened new avenues to probe the structures of protein assemblies in their near native states. Recent studies have begun applying single -particle analysis to heterogeneous mixtures, revealing the potential of structural-omics approaches that combine the power of mass spectrometry and electron microscopy. Here we highlight advances and challenges in sample preparation, data processing, and molecular modeling for handling increasingly comple ...[more]

PMID: 32129623

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