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A mouse model that is immunologically tolerant to reporter and modifier proteins.

ABSTRACT: Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8+ T cells specific for a model epitope, and substantially improves engraftment of reporter-gene transduced T cells. The Tol strain provides a valuable mouse model for cell transfer and viral-mediated gene transfer studies, and serves as a methodological example for the generation of poly-tolerant mouse strains.

SUBMITTER: Bresser K

PROVIDER: S-EPMC7260180 | biostudies-literature | 2020 May

REPOSITORIES: biostudies-literature

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A mouse model that is immunologically tolerant to reporter and modifier proteins.

Bresser Kaspar K Dijkgraaf Feline E FE Pritchard Colin E J CEJ Huijbers Ivo J IJ Song Ji-Ying JY Rohr Jan C JC Scheeren Ferenc A FA Schumacher Ton N TN

Communications biology 20200529 1

Reporter proteins have become an indispensable tool in biomedical research. However, exogenous introduction of these reporters into mice poses a risk of rejection by the immune system. Here, we describe the generation, validation and application of a multiple reporter protein tolerant 'Tol' mouse model that constitutively expresses an assembly of shuffled reporter proteins from a single open reading frame. We demonstrate that expression of the Tol transgene results in the deletion of CD8<sup>+</ ...[more]

PMID: 32472011

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