Ontology highlight
ABSTRACT:
SUBMITTER: Eun K
PROVIDER: S-EPMC7269240 | biostudies-literature | 2020
REPOSITORIES: biostudies-literature
Eun Kiyoung K Hong Nayoung N Jeong Yeon Woo YW Park Min Gi MG Hwang Seon-Ung SU Jeong Yeon I K YIK Choi Eun Ji EJ Olsson P Olof PO Hwang Woo Suk WS Hyun Sang-Hwan SH Kim Hyunggee H
PloS one 20200603 6
Recent advances in somatic cell nuclear transfer (SCNT) in canines facilitate the production of canine transgenic models. Owing to the importance of stable and strong promoter activity in transgenic animals, we tested human elongation factor 1α (hEF1α) and cytomegalovirus (CMV) promoter sequences in SCNT transgenic dogs. After transfection, transgenic donor fibroblasts with the hEF1α-enhanced green fluorescence protein (EGFP) transgene were successfully isolated using fluorescence-activated cell ...[more]