Ontology highlight
ABSTRACT:
SUBMITTER: He J
PROVIDER: S-EPMC7281462 | biostudies-literature | 2020 May
REPOSITORIES: biostudies-literature
He Jianlong J Liu Le L Liu Xiaoyan X Tang Kai K
Marine drugs 20200506 5
We cloned a xylanase gene (<i>xynT</i>) from marine bacterium <i>Echinicola rosea</i> sp. nov. JL3085<sup>T</sup> and recombinantly expressed it in <i>Escherichia coli</i> BL21. This gene encoded a polypeptide with 379 amino acid residues and a molecular weight of ~43 kDa. Its amino acid sequence shared 45.3% similarity with an endoxylanase from Cellvibrio mixtus that belongs to glycoside hydrolases family 10 (GH10). The XynT showed maximum activity at 40 °C and pH 7.0, and a maximum velocity of ...[more]