Unknown

Dataset Information

0

High-throughput intracellular biopsy of microRNAs for dissecting the temporal dynamics of cellular heterogeneity.


ABSTRACT: The capability to analyze small RNAs responsible for post-transcriptional regulation of genes expression is essential for characterizing cellular phenotypes. Here, we describe an intracellular biopsy technique (inCell-Biopsy) for fast, multiplexed, and highly sensitive profiling of microRNAs (miRNAs). The technique uses an array of diamond nanoneedles that are functionalized with size-dependent RNA binding proteins, working as "fishing rods" to directly pull miRNAs out of cytoplasm while keeping the cells alive, thus enabling quasi-single-cell miRNA analysis. Each nanoneedle works as a reaction chamber for parallel in situ amplification, visualization, and quantification of miRNAs as low as femtomolar, which is sufficient to detect miRNAs of a single-copy intracellular abundance with specificity to single-nucleotide variation. Using inCell-Biopsy, we analyze the temporal miRNA transcriptome over the differentiation of embryonic stem cells (ESCs). The combinatorial miRNA expression patterns derived by inCell-Biopsy identify emerging cell subpopulations differentiated from ESCs and reveal the dynamic evolution of cellular heterogeneity.

SUBMITTER: Wang Z 

PROVIDER: S-EPMC7286670 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC3090749 | biostudies-literature
| S-EPMC7273721 | biostudies-literature
| S-EPMC2323234 | biostudies-literature
| S-EPMC3589817 | biostudies-literature
2020-04-01 | GSE141030 | GEO
| S-EPMC9908848 | biostudies-literature
| S-EPMC8201685 | biostudies-literature
| S-EPMC5147906 | biostudies-literature
| S-EPMC7973443 | biostudies-literature
| S-EPMC6256031 | biostudies-literature