Spatially-resolved metabolic profiling of living Drosophila in neurodegenerative conditions using ¹H magic angle spinning NMR.

ABSTRACT: Drosophila flies are versatile animal models for the study of gene mutations in neuronal pathologies. Their small size allows performing in vivo Magic Angle Spinning (MAS) experiments to obtain high-resolution ¹H nuclear magnetic resonance (NMR) spectra. Here, we use spatially-resolved ¹H high-resolution MAS NMR to investigate in vivo metabolite contents in different segments of the fly body. A comparative study of metabolic changes was performed for three neurodegenerative disorders: two cell-specific neuronal and glial models of Huntington disease (HD) and a model of glutamate excitotoxicity. It is shown that these pathologies are characterized by specific and sometimes anatomically localized variations in metabolite concentrations. In two cases, the modifications of ¹H MAS NMR spectra localized in fly heads were significant enough to allow the creation of a predictive model.

SUBMITTER: Yon M 

PROVIDER: S-EPMC7289880 | biostudies-literature | 2020 Jun

REPOSITORIES: biostudies-literature