Ontology highlight
ABSTRACT:
SUBMITTER: Yang HQ
PROVIDER: S-EPMC7299345 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Yang Hua-Qian HQ Pérez-Hernández Marta M Sanchez-Alonso Jose J Shevchuk Andriy A Gorelik Julia J Rothenberg Eli E Delmar Mario M Coetzee William A WA
eLife 20200114
We investigated targeting mechanisms of Na<sup>+</sup> and K<sub>ATP</sub> channels to the intercalated disk (ICD) of cardiomyocytes. Patch clamp and surface biotinylation data show reciprocal downregulation of each other's surface density. Mutagenesis of the Kir6.2 ankyrin binding site disrupts this functional coupling. Duplex patch clamping and Angle SICM recordings show that I<sub>Na</sub> and I<sub>KATP</sub> functionally co-localize at the rat ICD, but not at the lateral membrane. Quantitat ...[more]